Podcasts for discipline microbiology (2)

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{"discipline":"microbiology-4zXyLE1Hr","podcasts":{"items":[{"ID":94,"status":40,"date_created":"2023-05-11 08:00:42","article_ID":94,"audience_type":0,"stats_view":482,"stats_like":3,"stats_save":0,"stats_play":0,"title":"Intercellular interaction mechanisms promote diversity in intracellular ATP concentration in Escherichia coli populations","abstract":"In fluctuating environments, many microorganisms acquire phenotypic heterogeneity as a survival tactic to increase the likelihood of survival of the overall population. One example of this interindividual heterogeneity is the diversity of ATP concentration among members of Escherichia coli populations under glucose deprivation. Despite the importance of such environmentally driven phenotypic heterogeneity, how the differences in intracellular ATP concentration emerge among individual E. coli organisms is unknown. In this study, we focused on the mechanism through which individual E. coli achieve high intracellular ATP concentrations. Through the use of microfluidic devices and cellular lineage analysis, we showed that the appearance of high ATP-holding individuals observed among E. coli can be explained only by intercellular transmission. By performing metabolomic analysis of post-culture medium, we revealed a significant increase in the ATP, especially at low glucose, and that the number of E. coli that retain significantly higher ATP can be controlled by adding large amounts of ATP to the medium, even in populations cultured under control glucose concentrations. These results reveal for the first time that ATP-mediated intercellular transmission enables some individuals in E. coli populations grown at low glucose to retain large amounts of ATP.","duration":null,"file_audio":null,"file_video":"1683791664689H3HUr.mp4","compressed":1,"slug":"intercellular-interaction-mechanisms-promote-diversity-in-intracellular-atp-concentration-in-escherichia-coli-populations-4zXyLE1I1","date_published_readable":"May 11, 2023","author":"Ryo Nakatani","author_slug":"ryo-nakatani-4zXyLE1K2","keywords":[{"name":"atp","slug":"atp-4zXyLE1Kp"},{"name":"lineage analysis","slug":"lineage-analysis-4zXyLE1Kq"},{"name":"e. coli","slug":"e-coli-4zXyLE1Kr"}],"disciplines":[]},{"ID":90,"status":40,"date_created":"2023-05-05 19:20:48","article_ID":90,"audience_type":0,"stats_view":68,"stats_like":0,"stats_save":0,"stats_play":0,"title":"Molecular characterization of Blastocystis subtypes in symptomatic patients from the southern region of Syria","abstract":"Blastocystis sp. is an intestinal parasite whose pathogenic role is underestimated. This parasite may be transmitted through food or water or by contact with human or animal feces. The role of Blastocystis in causing disease is controversial. Some subtypes of Blastocystis may be more likely to be associated with symptoms (Abdominal pain, Diarrhea, bloating..). This study is the first in Syria to identify and characterize Blastocystis sp. subtypes in individuals suffering from various gastrointestinal symptoms. Five different Blastocystis STs (ST1-ST5) had been identified where the ST1 was the most predominant. The individuals infected with ST1 had various gastrointestinal symptoms; however, no significant correlation was found between the different symptoms. We strongly recommend considering this parasite in laboratory reports, diagnosis, and treatment especially when no coinfection (no other intestinal parasite) is present.","duration":null,"file_audio":null,"file_video":"1683318873053lRmJb.mp4","compressed":1,"slug":"molecular-characterization-of-blastocystis-subtypes-in-symptomatic-patients-from-the-southern-region-of-syria-4zXyLE1HX","date_published_readable":"May 05, 2023","author":"samar al nahhas","author_slug":"samar-al-nahhas-4zXyLE1K9","keywords":[{"name":"blastocystis","slug":"blastocystis-4zXyLE1Ki"},{"name":"syria","slug":"syria-4zXyLE1Kj"},{"name":"gastrointestinal infections","slug":"gastrointestinal-infections-4zXyLE1Kk"}],"disciplines":[]}],"count":2},"head\/og\/url":"disciplines\/microbiology-4zXyLE1Hr","head\/title":"Podcasts for the discipline microbiology","head\/description":"Podcasts for the discipline microbiology","URL_MEDIA":"https:\/\/media.podscholars.com\/"}