lineage analysis (1 total items)

Recent uploads

There are no more podcasts to load.

Start sharing your research on PodScholars!

Upload a podcast

Start sharing your research on PodScholars!

Upload a podcast

{"keyword":"lineage-analysis-4zXyLE1Kq","podcasts":{"keyword":"lineage analysis","items":[{"ID":94,"status":40,"date_created":"2023-05-11 08:00:42","article_ID":94,"audience_type":0,"stats_view":482,"stats_like":3,"stats_save":0,"stats_play":0,"title":"Intercellular interaction mechanisms promote diversity in intracellular ATP concentration in Escherichia coli populations","abstract":"In fluctuating environments, many microorganisms acquire phenotypic heterogeneity as a survival tactic to increase the likelihood of survival of the overall population. One example of this interindividual heterogeneity is the diversity of ATP concentration among members of Escherichia coli populations under glucose deprivation. Despite the importance of such environmentally driven phenotypic heterogeneity, how the differences in intracellular ATP concentration emerge among individual E. coli organisms is unknown. In this study, we focused on the mechanism through which individual E. coli achieve high intracellular ATP concentrations. Through the use of microfluidic devices and cellular lineage analysis, we showed that the appearance of high ATP-holding individuals observed among E. coli can be explained only by intercellular transmission. By performing metabolomic analysis of post-culture medium, we revealed a significant increase in the ATP, especially at low glucose, and that the number of E. coli that retain significantly higher ATP can be controlled by adding large amounts of ATP to the medium, even in populations cultured under control glucose concentrations. These results reveal for the first time that ATP-mediated intercellular transmission enables some individuals in E. coli populations grown at low glucose to retain large amounts of ATP.","duration":null,"file_audio":null,"file_video":"1683791664689H3HUr.mp4","compressed":1,"slug":"intercellular-interaction-mechanisms-promote-diversity-in-intracellular-atp-concentration-in-escherichia-coli-populations-4zXyLE1I1","date_published_readable":"May 11, 2023","author":"Ryo Nakatani","author_slug":"ryo-nakatani-4zXyLE1K2","keywords":[{"name":"atp","slug":"atp-4zXyLE1Kp"},{"name":"lineage analysis","slug":"lineage-analysis-4zXyLE1Kq"},{"name":"e. coli","slug":"e-coli-4zXyLE1Kr"}],"disciplines":[]}],"count":1},"URL_MEDIA":"https:\/\/media.podscholars.com\/","head\/title":"Podcasts for keyword #lineage-analysis","head\/description":"Podcasts for keyword #lineage-analysis","head\/og\/url":"keywords\/lineage-analysis-4zXyLE1Kq"}