Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in /home/n6a57f5/podscholars.com/www/app/podcasts/m.PodcastsPublic.php on line 396

Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in /home/n6a57f5/podscholars.com/www/app/podcasts/m.PodcastsPublic.php on line 396

Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in /home/n6a57f5/podscholars.com/www/app/podcasts/m.PodcastsPublic.php on line 396

Deprecated: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated in /home/n6a57f5/podscholars.com/www/app/podcasts/m.PodcastsPublic.php on line 396
Intercellular interaction mechanisms promote diversity in intracellular ATP concentration in Escherichia coli populations | PodScholars.com

Intercellular interaction mechanisms promote diversity in intracellular ATP concentration in Escherichia coli populations

In fluctuating environments, many microorganisms acquire phenotypic heterogeneity as a survival tactic to increase the likelihood of survival of the overall population. One example of this interindividual heterogeneity is the diversity of ATP concentration among members of Escherichia coli populations under glucose deprivation. Despite the importance of such environmentally driven phenotypic heterogeneity, how the differences in intracellular ATP concentration emerge among individual E. coli organisms is unknown. In this study, we focused on the mechanism through which individual E. coli achieve high intracellular ATP concentrations. Through the use of microfluidic devices and cellular lineage analysis, we showed that the appearance of high ATP-holding individuals observed among E. coli can be explained only by intercellular transmission. By performing metabolomic analysis of post-culture medium, we revealed a significant increase in the ATP, especially at low glucose, and that the number of E. coli that retain significantly higher ATP can be controlled by adding large amounts of ATP to the medium, even in populations cultured under control glucose concentrations. These results reveal for the first time that ATP-mediated intercellular transmission enables some individuals in E. coli populations grown at low glucose to retain large amounts of ATP.

Posted on May 11, 2023

Reference
Authors
Publication Source
Scientific reports
Publication Date
May 2024

Document Object Identifier / Link
{"head\/og\/url":"podcasts\/intercellular-interaction-mechanisms-promote-diversity-in-intracellular-atp-concentration-in-escherichia-coli-populations-4zXyLE1I1","head\/title":"Intercellular interaction mechanisms promote diversity in intracellular ATP concentration in Escherichia coli populations","head\/description":"In fluctuating environments, many microorganisms acquire phenotypic heterogeneity as a survival tactic to increase the likelihood of survival of the overall population. One example of this interindividual heterogeneity is the diversity of ATP concentration among members of Escherichia coli populations under glucose deprivation. Despite the importance of such environmentally driven phenotypic heterogeneity, how the differences in intracellular ATP concentration emerge among individual E. coli organisms is unknown. In this study, we focused on the mechanism through which individual E. coli achieve high intracellular ATP concentrations. Through the use of microfluidic devices and cellular lineage analysis, we showed that the appearance of high ATP-holding individuals observed among E. coli can be explained only by intercellular transmission. By performing metabolomic analysis of post-culture medium, we revealed a significant increase in the ATP, especially at low glucose, and that the number of E. coli that retain significantly higher ATP can be controlled by adding large amounts of ATP to the medium, even in populations cultured under control glucose concentrations. These results reveal for the first time that ATP-mediated intercellular transmission enables some individuals in E. coli populations grown at low glucose to retain large amounts of ATP.","head\/og\/image":"https:\/\/media.podscholars.com\/f\/podcasts\/4zXyLE1I1.jpg","podcast":{"ID":94,"status":40,"date_created":"2023-05-11 08:00:42","owner_ID":219,"article_ID":94,"audience_type":0,"stats_view":431,"stats_like":3,"stats_save":0,"stats_play":0,"title":"Intercellular interaction mechanisms promote diversity in intracellular ATP concentration in Escherichia coli populations","abstract":"In fluctuating environments, many microorganisms acquire phenotypic heterogeneity as a survival tactic to increase the likelihood of survival of the overall population. One example of this interindividual heterogeneity is the diversity of ATP concentration among members of Escherichia coli populations under glucose deprivation. Despite the importance of such environmentally driven phenotypic heterogeneity, how the differences in intracellular ATP concentration emerge among individual E. coli organisms is unknown. In this study, we focused on the mechanism through which individual E. coli achieve high intracellular ATP concentrations. Through the use of microfluidic devices and cellular lineage analysis, we showed that the appearance of high ATP-holding individuals observed among E. coli can be explained only by intercellular transmission. By performing metabolomic analysis of post-culture medium, we revealed a significant increase in the ATP, especially at low glucose, and that the number of E. coli that retain significantly higher ATP can be controlled by adding large amounts of ATP to the medium, even in populations cultured under control glucose concentrations. These results reveal for the first time that ATP-mediated intercellular transmission enables some individuals in E. coli populations grown at low glucose to retain large amounts of ATP.","duration":null,"file_audio":null,"file_video":"1683791664689H3HUr.mp4","compressed":1,"author_firstname":"Ryo","author_lastname":"Nakatani","article_date":2022,"article_DOI":"https:\/\/doi.org\/10.1038\/s41598-022-22189-x","publication_ID":29,"publication_name":"Scientific reports","author_slug":"ryo-nakatani-4zXyLE1K2","publication_slug":"scientific-reports-4zXyLE1GY","keywords":[{"name":"atp","slug":"atp-4zXyLE1Kp"},{"name":"lineage analysis","slug":"lineage-analysis-4zXyLE1Kq"},{"name":"e. coli","slug":"e-coli-4zXyLE1Kr"}],"disciplines":[{"name":"Microbiology","slug":"microbiology-4zXyLE1Hr"}],"publication_authors":[{"name":"Ryo Nakatani","slug":"ryo-nakatani-4zXyLE1K2"},{"name":"Takahiro Yamada","slug":"takahiro-yamada-4zXyLE1Kg"},{"name":"Norkio Hiroi","slug":"norkio-hiroi-4zXyLE1Kh"},{"name":"Akira Funahashi","slug":"akira-funahashi-4zXyLE1Ki"}],"authors_links":[],"slug":"intercellular-interaction-mechanisms-promote-diversity-in-intracellular-atp-concentration-in-escherichia-coli-populations-4zXyLE1I1"}}

Error

Message: str_replace(): Passing null to parameter #3 ($subject) of type array|string is deprecated
Type: 8192
File: /home/n6a57f5/podscholars.com/www/app/podcasts/m.PodcastsPublic.php
Line: 396


Stack trace